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Gomes,Bruna C.; Martinis,Elaine C.P. de. |
Helicobacter pylori is known, worldwide, as the causative agent of gastric diseases. However, its transmission route has not been completely understood. To evaluate the survival of H. pylori (a clinical and a reference strain) artificially inoculated on lettuce and carrot samples, portions of 25 g were inoculated with approximately 10(6) CFU/g of H. pylori and packed under normal and/or modified atmosphere (3% oxygen, 10% carbon dioxide, and 87% nitrogen). The inoculated food samples were stored at 8ºC, with daily enumeration of H. pylori populations on Columbia blood agar (CBA) and/ or Helicobacter pylori Special Peptone Agar (HPSPA). When CBA with antibiotics was used, the clinical isolate H. pylori HP1 was detected for up to 72 h in sanitized lettuce... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: H. pylori; Peptic ulcer; Modified atmosphere; Vegetable. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100024 |
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Zabaglia,Luanna Munhoz; Ferraz,Mariane Avante; Pereira,Weendelly Nayara; Orcini,Wilson Aparecido; Labio,Roger Willian de; Caleman Neto,Agostinho; Wisnieski,Fernanda; Oliveira,Juliana Garcia de; Smith,Marilia de Arruda Cardoso; Payão,Spencer Luiz Marques; Rasmussen,Lucas Trevizani. |
Abstract Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF-α gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: H. pylori; TNF-α Gene expression; Gastric cancer cagA. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100355 |
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Lyra,Andre C.; Santana,Genoile; Santana,Nelma; Silvany-Neto,Anibal; Magalhães,Emilia; Pereira,Eduardo M.; Mascarenhas,Ramiro; Lyra,Marcos C.; Veiga,Andrea; Ferreira,Karina; Zaterka,Schilioma; Lyra,Luiz G.. |
Helicobacter pylori plays an important role in the etiology of peptic ulcer disease. Its prevalence appears to be higher in developing countries. We evaluated the seroprevalence of H. pylori and risk factors associated with infection in voluntary blood donors who attended the main blood center of the city of Salvador, Brazil. The subjects responded to an epidemiological questionnaire, with information about sex, age, race, lifestyle, social-economic level indicators, and residence and hygiene conditions. Anti-H. pylori antibody was determined by ELISA (Cobas Core, Roche). Three hundred and seven subjects were included in the study. Anti-H. pylori antibody results were indeterminate in 33 individuals (10.8%), who were excluded from analysis. Among the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: H. pylori; Seroprevalence; Risk factors; Brazil. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500009 |
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